D-stem mutation in an essential tRNA increases translation speed at the cost of fidelity.
The efficiency with which aminoacyl-tRNA and GTP-bound translation elongation factor EF-Tu recognizes the A-site codon of the ribosome is dependent on codons and tRNA species present in the polypeptide (P) and exit (E) Protein Snacks codon sites.To understand how codon context affects the efficiency of codon recognition by tRNA-bound EF-Tu, a genet